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Journal Article 14

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in vitro digestion 1

in vitro fertilization 1

in vitro imaging 1

Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis 1

DFT study 1

Ginkgo bilobaextract 1

Ginkgolide B 1

Ginsenoside CK 1

Icraiin 1

MID2 1

PCOS 1

Propionibacterium acnes 1

acetyl salicylic acid 1

acute ischemic stroke 1

antimicrobial susceptibility/resistance 1

artificial gametes 1

artificial ovary 1

artificial uterus 1

assisted reproduction 1

biochip 1

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Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical

Ying Ma,Nanxue Zhang,Shi Wu,Haihui Huang,Yanpei Cao

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 517-521 doi: 10.1007/s11684-016-0480-9

Abstract:

This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients (70.41%). The susceptibility to triple antibiotic ointment (neomycin/bacitracin/polymyxin B) and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.

Keywords： antimicrobial susceptibility/resistance     Propionibacterium acnes     topical antibiotics     in vitro study    

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In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 1,   Pages 69-78 doi: 10.1007/s11783-013-0502-7

Abstract: In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic

Keywords： waste water     in vitro     recombinant yeast assay     ethoxyresorufin-O-deethylase (EROD)     receptor     endocrine    

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and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro

Lan Wang,Jueheng Wu,Jie Yuan,Xun Zhu,Hongmei Wu,Mengfeng Li

Frontiers of Medicine 2016, Volume 10, Issue 1,   Pages 41-51 doi: 10.1007/s11684-016-0429-z

Abstract: siRNA silencing of MID2 expression significantly reduced MCF-7 and MDA-MB-231 cell proliferation in vitro

Keywords： breast cancer     MID2     proliferation     overall survival     xenograft    

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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 4,   Pages 635-644 doi: 10.15302/J-FASE -2020343

Abstract:

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

 

Keywords： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis    

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extract and Ginkgolide B against oxygen–glucose deprivation/reoxygenation and glucose injury in a new in vitro

Xiaohan Yang, Tiezheng Zheng, Hao Hong, Nan Cai, Xiaofeng Zhou, Changkai Sun, Liying Wu, Shuhong Liu, Yongqi Zhao, Lingling Zhu, Ming Fan, Xuezhong Zhou, Fengxie Jin

Frontiers of Medicine 2018, Volume 12, Issue 3,   Pages 307-318 doi: 10.1007/s11684-017-0547-2

Abstract:

Acute ischemic stroke (AIS), as the third leading cause of death worldwide, is characterized by its high incidence, mortality rate, high incurred disability rate, and frequent reoccurrence. The neuroprotective effects of extract (GBE) against several cerebral diseases have been reported in previous studies, but the underlying mechanisms of action are still unclear. Using a novel rat cortical capillary endothelial cell-astrocyte-neuron network model, we investigated the neuroprotective effects of GBE and one of its important constituents, Ginkgolide B (GB), against oxygen–glucose deprivation/reoxygenation and glucose (OGD/R) injury. In this model, rat cortical capillary endothelial cells, astrocytes, and neurons were cocultured so that they could be synchronously observed in the same system. Pretreatment with GBE or GB increased the neuron cell viability, ameliorated cell injury, and inhibited the cell apoptotic rate through Bax and Bcl-2 expression regulation after OGD/R injury. Furthermore, GBE or GB pretreatment enhanced the transendothelial electrical resistance of capillary endothelial monolayers, reduced the endothelial permeability coefficients for sodium fluorescein (Na-F), and increased the expression levels of tight junction proteins, namely, ZO-1 and occludin, in endothelial cells. Results demonstrated the preventive effects of GBE on neuronal cell death and enhancement of the function of brain capillary endothelial monolayers after OGD/R injury ; thus, GBE could be used as an effective neuroprotective agent for AIS/reperfusion, with GB as one of its significant constituents.

Keywords： acute ischemic stroke     Ginkgo bilobaextract     Ginkgolide B     network model     neuroprotection    

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Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 106-115 doi: 10.15302/J-FASE-2017133

Abstract: A series of dehydroabietic acid-based diarylamines have been synthesized in order to investigate their fluorescent properties, photostability, cell toxicity and fluorescence imaging. The geometries as well as their molecular properties were optimized at the B3LYP/6-31G* level using Gaussian 03. The results indicate that molecular geometry, HOMO and LUMO energies, and energy gaps are important to predict absorption and fluorescent properties. Five of the compounds can be effectively taken up by human cervical carcinoma, human hepatocellular carcinoma SMMC-7721, human gastric cancer SGC-7901 and human lung adenocarcinoma A549 cells and strong blue fluorescent signals are detected in these cells. These compounds are potential candidates for fluorescent probes in biological diagnosis.

Keywords： dehydroabietic acid-based diarylamine     DFT study     fluorescent probe     in vitro imaging    

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Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems

Ranvijay KUMAR, Kaushlendra SHARMA

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 1,   Pages 116-122 doi: 10.1007/s11705-013-1310-0

Abstract: salicylic acid was incorporated in the various Psy- -PMA samples and tablets were prepared to study the in vitro

Keywords： psyllium     acetyl salicylic acid     in-vitro drug release     swelling     biodegradation    

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Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu, Lei Jin

Frontiers of Medicine 2017, Volume 11, Issue 2,   Pages 247-252 doi: 10.1007/s11684-017-0519-6

Abstract: The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro

Keywords： in vitro fertilization     PCOS     free radical     embryo quality    

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Implications of static

John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 340-350 doi: 10.15302/J-FASE-2018209

Abstract: To test the efficacy of these different fibers on starch digestion, static in vitro digestionIndeed, many efforts have been committed to the development of various static in vitro protocolsThis summary can benefit investigators in developing static in vitro digestion models designed

Keywords： dietary fiber     in vitro digestion     nutrition     simulation     starch    

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Strategic Research on the Molecular Diagnostics Industry in China

Xie Lan,Liu Ran,Feng Juan,Xing Wanli and Cheng Jing

Strategic Study of CAE 2017, Volume 19, Issue 2,   Pages 29-36 doi: 10.15302/J-SSCAE-2017.02.005

Abstract: diagnostics is an important part of personalized medicine and has become the main driving force of the in vitro

Keywords： molecular diagnostics     in vitro diagnostics     market analysis     policies and regulations     biochip    

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What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations

Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 627-638 doi: 10.1007/s11684-019-0697-5

Abstract: Assisted reproduction provides a wide spectrum of treatments and strategies addressing infertility. However, distinct groups of infertile patients with unexplained infertility, congenital disorders, and other complex cases pose a challenge in fertilization (IVF) practices. This special cohort of patients is associated with futile attempts, IVF overuse, and dead ends in management. Cutting edge research on animal models introduced this concept, along with the development of artificial organs with the aim to mimic the respective physiological functions in reproduction. Extrapolation on clinical application leads to the future use of infertility management in humans. To date, the successful clinical application of artificial reproductive organs in humans is not feasible because further animal model studies are required prior to clinical trials. The application of these artificial organs could provide a solution to infertility cases with no other options. This manuscript presents an overview on the current status, future prospects, and considerations on the potential clinical application of artificial ovary, uterus, and gametes in humans. This paper presents how the IVF practice landscape may be shaped and challenged in the future, along with the subsequent concerns in assisted reproductive treatments.

Keywords： artificial ovary     artificial uterus     artificial gametes     assisted reproduction     considerations     in vitro    

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Effects of red and blue LEDs on

Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 2,   Pages 197-205 doi: 10.15302/J-FASE-2018224

Abstract: The objectives of this study were to investigate the effects of red and blue LEDs on growth and microtuberization of potato ( ) single-node cuttings. Explants were incubated under 6 light treatments: 100% red LEDs (R), 75% red LEDs+ 25% blue LEDs (3RB), 50% red LEDs+ 50% blue LEDs (RB), 25% red LEDs+ 75% blue LEDs (R3B), 100% blue LEDs (B) and white LEDs (W). Most of the growth and physiological parameters were significantly higher in 3RB than W. Enhancement of leaf area and chlorophyll concentrations were obtained in B. Leaf stomata were elliptical with the lowest density in 3RB. However, those in W were round in shape, and those with the smallest size and the highest density were observed in R. Most of the characteristics of microtuberization were also improved in 3RB. The combined spectra of red and blue LEDs increased the number of large microtubers. The fresh weight of individual microtubers in R and W were increased, but not their number. These results suggest that, of the treatments assessed, 3RB is optimal for the growth of potato plantlets and the combination of red and blue LEDs is beneficial for microtuberization.

Keywords： blue LED     microtuber     plant tissue culture     potato plantlets in vitro     red LED    

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The lethal dose test of Ginsenoside CK and Icraiin for sperm in vitro

Yu Hongliang,Chang Mingxiu,Cao Henghai,Bo Liwei,Yang Yongjun,Li Chongmin,Li Ming

Strategic Study of CAE 2015, Volume 17, Issue 6,   Pages 86-88

Abstract:

Objective：To investigate the effects of Ginsenoside CK(Gin CK),Icraiin(Icr) for sperm survive in vitroMethods：40 sample of male semen were cultured in medium and grouped it in vitro,To adjust the semen concentration

Keywords： Ginsenoside CK     Icraiin     sperm     lethal dose    

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Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in autumn in Beijing, China

LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 3,   Pages 433-440 doi: 10.1007/s11783-013-0594-0

Abstract: sludge samples collected from 17 different sewage treatment plants in Beijing, based on a batch of in vitro

Keywords： sewage sludge     in vitro bioassay     endocrine disruption     dioxin-like effect    

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Title Author Date Type Operation

Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical

Ying Ma,Nanxue Zhang,Shi Wu,Haihui Huang,Yanpei Cao

Journal Article

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

Journal Article

and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro

Lan Wang,Jueheng Wu,Jie Yuan,Xun Zhu,Hongmei Wu,Mengfeng Li

Journal Article

IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Journal Article

extract and Ginkgolide B against oxygen–glucose deprivation/reoxygenation and glucose injury in a new in vitro

Xiaohan Yang, Tiezheng Zheng, Hao Hong, Nan Cai, Xiaofeng Zhou, Changkai Sun, Liying Wu, Shuhong Liu, Yongqi Zhao, Lingling Zhu, Ming Fan, Xuezhong Zhou, Fengxie Jin

Journal Article

Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

Journal Article

Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems

Ranvijay KUMAR, Kaushlendra SHARMA

Journal Article

Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu, Lei Jin

Journal Article

Implications of static

John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG

Journal Article

Strategic Research on the Molecular Diagnostics Industry in China

Xie Lan,Liu Ran,Feng Juan,Xing Wanli and Cheng Jing

Journal Article

What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations

Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris

Journal Article

Effects of red and blue LEDs on

Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU

Journal Article

The lethal dose test of Ginsenoside CK and Icraiin for sperm in vitro

Yu Hongliang,Chang Mingxiu,Cao Henghai,Bo Liwei,Yang Yongjun,Li Chongmin,Li Ming

Journal Article

Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in autumn in Beijing, China

LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan

Journal Article